Two-Dimensional Gel Electrophoresis of Ribosomal Proteins from Streptomycin-Sensitive and Streptomycin-Resistant Mutants of Chlamydomonas reinhardi

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Studies on the origin of streptomycin resistant mutants in Chlamydomonas reinhardi.

HE inheritance of mutations to streptomycin resistance in Chlamydomonas Treinhardi was first described by SAGER (1954) who showed that two types of streptomycin resistant mutations occur in this organism and that each type possesses a different pattern of inheritance. Mutations of the first type, designated sr-l or sr-100, are resistant to 100 pg per ml of streptomycin. These mutant strains exh...

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Streptomycin induced protein expression analysis in Mycobacterium tuberculosis by two-dimensional gel electrophoresis & mass spectrometry.

BACKGROUND & OBJECTIVES The resistance of Mycobacterium tuberculosis to streptomycin, a core drug for treatment of category II tuberculosis (TB) has posed a major challenge to the health providers as well as research workers worldwide and has severely compromised the therapeutic options. A significant proportion of streptomycin resistant M. tuberculosis isolates failed to show mutations in conv...

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Since Gorini and Katajal found that streptomycin can phenotypically suppress (cure) auxotrophic mutations in streptomycin-resistant strains of Escherichia coli, the ribosome has been implicated in genotypic suppression. However, the role of mutations in the str locus in curing and suppression has remained unclear. In particular, it is unknown whether a mutation to str-R (see ref. 2 for terminol...

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ژورنال

عنوان ژورنال: European Journal of Biochemistry

سال: 1975

ISSN: 0014-2956,1432-1033

DOI: 10.1111/j.1432-1033.1975.tb02411.x